TY - JOUR
T1 - Rat interleukin-18 binding protein
T2 - Cloning, expression, and characterization
AU - Im, Sin Hyeog
AU - Kim, Soo Hyun
AU - Azam, Tania
AU - Venkatesh, Natarajan
AU - Dinarello, Charles A.
AU - Fuchs, Sara
AU - Souroujon, Miriam C.
PY - 2002
Y1 - 2002
N2 - Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-γ (IFN-γ) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.
AB - Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-γ (IFN-γ) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.
UR - http://www.scopus.com/inward/record.url?scp=0036013761&partnerID=8YFLogxK
U2 - 10.1089/107999002753675749
DO - 10.1089/107999002753675749
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C2 - 12034039
AN - SCOPUS:0036013761
SN - 1079-9907
VL - 22
SP - 321
EP - 328
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 3
ER -