On the Interaction of Nucleotides with Poly-L-lysine and Poly-L-arginine. I. The Influence of the Nucleotide Base on the Binding Behavior

The binding of 5′-ribo- and 5′-deoxyribo-nucleoside monophosphates by poly-L-lysine and poly- L-arginine has been studied by the equilibrium dialysis technique. The different nucleotides were compared under standard conditions, in order to determine the influence of nucleotide bases on the binding parameters. It was found that the binding behavior is indeed dependent of the nature of the base; whereas the individual pyrimidine nucleotides exhibit little or no differences, the purine nucleotides exhibit large differences among each other and differ substantially from the pyrimidine nucleotides. In every case guanylic acid was found to bind most strongly. Reasons for observed differences in the binding behavior are discussed.