Cytonuclear interactions modulate the plasticity of photosynthetic rhythmicity and growth in wild barley

Lalit Dev Tiwari, Eyal Bdolach, Manas Ranjan Prusty, Schewach Bodenheimer, Avital Be'ery, Adi Faigenboim-Doron, Eiji Yamamoto, Klára Panzarová, Khalil Kashkush, Noam Shental, Eyal Fridman

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

In plants, the contribution of the plasmotype (mitochondria and chloroplast) in controlling the circadian clock plasticity and possible consequences on cytonuclear genetic makeup have yet to be fully elucidated. A genome-wide association study in the wild barley (Hordeum vulgare ssp. spontaneum) B1K collection identified overlap with our previously mapped DRIVERS OF CLOCKS (DOCs) loci in wild-cultivated interspecific population. Moreover, we identified non-random segregation and epistatic interactions between nuclear DOCs loci and the chloroplastic RpoC1 gene, indicating an adaptive value for specific cytonuclear gene combinations. Furthermore, we show that DOC1.1, which harbours the candidate SIGMA FACTOR-B (SIG-B) gene, is linked with the differential expression of SIG-B and CCA1 genes and contributes to the circadian gating response to heat. High-resolution temporal growth and photosynthesis measurements of B1K also link the DOCs loci to differential growth, Chl content and quantum yield. To validate the involvement of the Plastid encoded polymerase (PEP) complex, we over-expressed the two barley chloroplastic RpoC1 alleles in Arabidopsis and identified significant differential plasticity under elevated temperatures. Finally, enhanced clock plasticity of de novo ENU (N-Ethyl-N-nitrosourea) -induced barley rpoB1 mutant further implicates the PEP complex as a key player in regulating the circadian clock output. Overall, this study highlights the contribution of specific cytonuclear interaction between rpoC1 (PEP gene) and SIG-B with distinct circadian timing regulation under heat, and their pleiotropic effects on growth implicate an adaptive value.

שפה מקוריתאנגלית
מספר המאמרe14192
עמודים (מ-עד)e14192
כתב עתPhysiologia Plantarum
כרך176
מספר גיליון1
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 1 ינו׳ 2024

הערה ביבליוגרפית

Publisher Copyright:
© 2024 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.

© 2024 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.

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להלן מוצגים תחומי המחקר של הפרסום 'Cytonuclear interactions modulate the plasticity of photosynthetic rhythmicity and growth in wild barley'. יחד הם יוצרים טביעת אצבע ייחודית.

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