TY - JOUR
T1 - The incorporation of isotopically labelled glycine into glutathione of erythrocytes with glucose-6-phosphate dehydrogenase deficiency
AU - Szeinberg, Aryeh
AU - Adam, Avinoam
AU - Ramot, Bracha
AU - Sheba, Chaim
AU - Myers, Fanny
N1 - Funding Information:
This investigationh as been supportedb y grants from the Blood Research FoundationW, ashingtonD, .C., and from the RockefelleFr oundation.
PY - 1959/11
Y1 - 1959/11
N2 - 1. 1. Cysteine decreases the reduced glutathione level in normal red blood cells incubated under aerobic conditions in a glucose-free incubation medium. 2. 2. Cysteine has a similar effect in cells deficient in glucose-6-phosphate dehydrogenase, even in the presence of glucose. 3. 3. Investigations of the rate of glycine incorporation into glutathione of normal erythrocytes under aerobic conditions should be conducted in the presence of glucose when the incubation mixture contains cysteine. The glucose may be replaced by fructose, galactose or inosine. Cysteine should be omitted altogether from the incubation mixture when cells deficient in glucose-6-phosphate dehydrogenase are investigated. 4. 4. The rate of incorporation of glycine into glutathione in deficient cells was found to be lower than in normal cells. The possible causes of this difference are discussed.
AB - 1. 1. Cysteine decreases the reduced glutathione level in normal red blood cells incubated under aerobic conditions in a glucose-free incubation medium. 2. 2. Cysteine has a similar effect in cells deficient in glucose-6-phosphate dehydrogenase, even in the presence of glucose. 3. 3. Investigations of the rate of glycine incorporation into glutathione of normal erythrocytes under aerobic conditions should be conducted in the presence of glucose when the incubation mixture contains cysteine. The glucose may be replaced by fructose, galactose or inosine. Cysteine should be omitted altogether from the incubation mixture when cells deficient in glucose-6-phosphate dehydrogenase are investigated. 4. 4. The rate of incorporation of glycine into glutathione in deficient cells was found to be lower than in normal cells. The possible causes of this difference are discussed.
UR - http://www.scopus.com/inward/record.url?scp=49749207635&partnerID=8YFLogxK
U2 - 10.1016/0006-3002(59)90070-8
DO - 10.1016/0006-3002(59)90070-8
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C2 - 13836341
AN - SCOPUS:49749207635
SN - 0006-3002
VL - 36
SP - 65
EP - 73
JO - BBA - Biochimica et Biophysica Acta
JF - BBA - Biochimica et Biophysica Acta
IS - 1
ER -