Our hypothesis that inflammation in asthma involves production of ozone by white blood cells and that ozone could be an inflammatory mediator suggests that scavengers of reactive oxygen species (ROS), for example, electron-rich olefins, could serve for prophylactic treatment of asthma. Olefins could provide chemical protection against either exogenous or endogenous ozone and other ROS. BALB/c mice pretreated by inhalation of d-limonene before an ovalbumin challenge exhibited significant attenuation of the allergic asthma symptoms. Diminution of the inflammatory process was evident by reduced levels of aldehydes, reduced counts of neutrophils in the BAL fluid and by histological tests. A surprising systemic effect was observed by decreased levels of aldehydes in the spleen, suggesting that the examination of tissues and organs that are remote from the inflammation foci could provide valuable information on the distribution of the oxidative stress and may serve as guide for targeted treatment.
|Number of pages||5|
|Journal||Bioorganic and Medicinal Chemistry Letters|
|State||Published - 15 Jan 2015|
Bibliographical noteFunding Information:
We thank Dr. Y. Bar-Ilan of Migal—Galilee Research Institute for technical assistance and analytical input. This study was supported by NOFAR program issued through MAGNET directorate in the Israeli Ministry of Industry , Trade & Labor , Grant No. 35964 (E.K.); and by the League for Prevention of Lung Disease in Haifa (H.B.) and the E. and S. Kronvet Medical Research Fund (H.B.). E.K. is the incumbent of the Benno Gitter & Ilana Ben-Ami Chair of Biotechnology, Technion.
© 2014 Elsevier Ltd. All rights reserved.
- Aldehyde biomarkers
- Respiratory inflammation
- Systemic oxidative stress