Differences between embryonic and adult Torpedo acetylcholine receptor γ subunit

Miriam C. Souroujon; Sara Carmon; Anat Safran; Sara Fuchs

doi:10.1016/0014-5793(91)81039-B

Differences between embryonic and adult Torpedo acetylcholine receptor γ subunit

Miriam C. Souroujon, Sara Carmon, Anat Safran, Sara Fuchs

Life Sciences discipline

Research output: Contribution to journal › Article › peer-review

Abstract

Antibodies to a synthetic peptide corresponding to residues 346-359 of the Torpedo acetylcholine receptor (AChR) γ subunit, were employed to compare the adult and embryonic receptor. This peptide contains a consensus phosphorylation site for cAMP-dependent protein kinase (PKA). The anti-peptide antibodies discriminated between adult and embryonic AChRs, and reacted preferentially with the adult γ form. These observed immunological differences did not seem to stem from different phosphorylation states. Our results suggest that the embryonic Torpedo AChR may have a γ-like subunit that differs from the known adult form of this subunit, at least in the specific region that contains the phosphorylation site for PKA.

Original language	English
Pages (from-to)	222-226
Number of pages	5
Journal	FEBS Letters
Volume	288
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)81039-B
State	Published - 19 Aug 1991

Bibliographical note

Funding Information:
Acknowledgements: This work was supported by grants from the Association FrancaiseC ontre Its Myopathies( AFM), the Los Angeles Chapter of the Myasthenia Gravis Foundation, the Muscular Dystrophy Association of America, the United Stt!cs-Israel Binational Science Foundation and the Schilling Foundation.

Keywords

Adult and embryonic acetylcholine receptor
Anti-peptide antibody
Phosphorylation
γ Subunit

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10.1016/0014-5793(91)81039-B

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title = "Differences between embryonic and adult Torpedo acetylcholine receptor γ subunit",

abstract = "Antibodies to a synthetic peptide corresponding to residues 346-359 of the Torpedo acetylcholine receptor (AChR) γ subunit, were employed to compare the adult and embryonic receptor. This peptide contains a consensus phosphorylation site for cAMP-dependent protein kinase (PKA). The anti-peptide antibodies discriminated between adult and embryonic AChRs, and reacted preferentially with the adult γ form. These observed immunological differences did not seem to stem from different phosphorylation states. Our results suggest that the embryonic Torpedo AChR may have a γ-like subunit that differs from the known adult form of this subunit, at least in the specific region that contains the phosphorylation site for PKA.",

keywords = "Adult and embryonic acetylcholine receptor, Anti-peptide antibody, Phosphorylation, γ Subunit",

author = "Souroujon, {Miriam C.} and Sara Carmon and Anat Safran and Sara Fuchs",

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AU - Carmon, Sara

AU - Safran, Anat

AU - Fuchs, Sara

N1 - Funding Information: Acknowledgements: This work was supported by grants from the Association FrancaiseC ontre Its Myopathies( AFM), the Los Angeles Chapter of the Myasthenia Gravis Foundation, the Muscular Dystrophy Association of America, the United Stt!cs-Israel Binational Science Foundation and the Schilling Foundation.

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AB - Antibodies to a synthetic peptide corresponding to residues 346-359 of the Torpedo acetylcholine receptor (AChR) γ subunit, were employed to compare the adult and embryonic receptor. This peptide contains a consensus phosphorylation site for cAMP-dependent protein kinase (PKA). The anti-peptide antibodies discriminated between adult and embryonic AChRs, and reacted preferentially with the adult γ form. These observed immunological differences did not seem to stem from different phosphorylation states. Our results suggest that the embryonic Torpedo AChR may have a γ-like subunit that differs from the known adult form of this subunit, at least in the specific region that contains the phosphorylation site for PKA.

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Differences between embryonic and adult Torpedo acetylcholine receptor γ subunit

Abstract

Bibliographical note

Keywords

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