Antigenic specificity of acetylcholine receptor in developing muscle. Studies with monoclonal antibodies

Miry C. Souroujon, Sergio Pizzighella, Daria Mochly-Rosen, Sara Fuchs

Research output: Contribution to journalArticlepeer-review


Monoclonal antibodies (mcAbs) elicited against the nicotinic acetylcholine receptor (AChR) from Torpedo, were used to follow antigenic changes in AChR during muscle development. Newborn rat muscle and denervated mouse muscle were used as sources of extrajunctional AChR; adult innervated rat and mouse muscle were used as sources of junctional AChR. Most of the mcAbs tested reacted preferably, but not exclusively with extrajunctional AChR (EJR), as compared to junctional AChR (JR). None was found to react with only one of the two forms of AChR. We conclude that the anti-AChR monoclonal antibodies used in this study detect antigenic determinants which are shared by EJR and JR, but which probably undergo structural changes during muscle development.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalJournal of Neuroimmunology
Issue numberC
StatePublished - 1985
Externally publishedYes

Bibliographical note

Funding Information:
Acetylcholine receptors (AChRs) in normal adult skeletal muscle are densely packed at subsynaptic areas of the postsynaptic membrane. On the other hand, in This work was supported by the Muscular Dystrophy Association of America, the Los Angeles Chapter of the Myasthenia Gravis Foundation and the United States-Israel Binational Science Foundation (BSF). Dr. Pizzighella from the Department of Immunopathology,U niversity of Verona, was partly supported by a grant from Istituti Ospitalieri di Verona, Italy.


  • Acetylcholine receptor
  • Monoclonal antibodies
  • Muscle development


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