Rat interleukin-18 binding protein: Cloning, expression, and characterization

Sin Hyeog Im; Soo Hyun Kim; Tania Azam; Natarajan Venkatesh; Charles A. Dinarello; Sara Fuchs; Miriam C. Souroujon

doi:10.1089/107999002753675749

Rat interleukin-18 binding protein: Cloning, expression, and characterization

Sin Hyeog Im
, Soo Hyun Kim
, Tania Azam
, Natarajan Venkatesh
, Charles A. Dinarello
, Sara Fuchs
, Miriam C. Souroujon

מדעי החיים

نتاج البحث: نشر في مجلة › مقالة › مراجعة النظراء

ملخص

Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-γ (IFN-γ) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.

اللغة الأصلية	الإنجليزيّة
الصفحات (من إلى)	321-328
عدد الصفحات	8
دورية	Journal of Interferon and Cytokine Research
مستوى الصوت	22
رقم الإصدار	3
المعرِّفات الرقمية للأشياء	https://doi.org/10.1089/107999002753675749
حالة النشر	نُشِر - 2002

الوصول إلى المستند

10.1089/107999002753675749

الملفات والروابط الأخرى

Link to publication in Scopus

قم بذكر هذا

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title = "Rat interleukin-18 binding protein: Cloning, expression, and characterization",

abstract = "Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-γ (IFN-γ) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.",

author = "Im, \{Sin Hyeog\} and Kim, \{Soo Hyun\} and Tania Azam and Natarajan Venkatesh and Dinarello, \{Charles A.\} and Sara Fuchs and Souroujon, \{Miriam C.\}",

year = "2002",

doi = "10.1089/107999002753675749",

language = "אנגלית",

volume = "22",

pages = "321--328",

journal = "Journal of Interferon and Cytokine Research",

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TY - JOUR

T1 - Rat interleukin-18 binding protein

T2 - Cloning, expression, and characterization

AU - Im, Sin Hyeog

AU - Kim, Soo Hyun

AU - Azam, Tania

AU - Venkatesh, Natarajan

AU - Dinarello, Charles A.

AU - Fuchs, Sara

AU - Souroujon, Miriam C.

PY - 2002

Y1 - 2002

N2 - Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-γ (IFN-γ) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.

AB - Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-γ (IFN-γ) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.

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DO - 10.1089/107999002753675749

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AN - SCOPUS:0036013761

SN - 1079-9907

VL - 22

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EP - 328

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

IS - 3

ER -

Rat interleukin-18 binding protein: Cloning, expression, and characterization

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الملفات والروابط الأخرى

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