TY - JOUR
T1 - IONIC CHANNELS IN SYNAPTIC VESICLES
T2 - ARE THEY INVOLVED IN TRANSMITTER RELEASE?
AU - Rahamimoff, Rami
AU - Yakir, Nilly
AU - DeRiemer, Susan A.
AU - Ginsburg, Simona
AU - Kaiserman, Igor
AU - Shapira, Roy
AU - Sakamann, Bert
AU - Stadler, Herbert
PY - 1989/11/12
Y1 - 1989/11/12
N2 - Synaptic vesicles were isolated from the nerve terminals of Torpedo electric organ. After fusion, ‘giant’ vesicles were formed which could be examined by the patch clamp technique. One of the cationic channels, the P channel, shows a small preference for K+ compared to Na+ and has multiple conductance levels. Its rate of opening is voltage and calcium dependent. Fractal analysis of the P channels reveals that its behaviour does not seem to be fractal in nature. At voltages where only one conductance level is observed, fractal analysis shows at least one discrete open state and at least two discrete closed states. There are considerable similarities between the P channel and channels found in granules from the hypophysis. These channels resemble, in turn, the channels found in gap junctions. Therefore, it is not unwarranted to speculate that a gap‐junction‐like communication between the secretory vesicle and the extracellular space may occur during exocytosis.
AB - Synaptic vesicles were isolated from the nerve terminals of Torpedo electric organ. After fusion, ‘giant’ vesicles were formed which could be examined by the patch clamp technique. One of the cationic channels, the P channel, shows a small preference for K+ compared to Na+ and has multiple conductance levels. Its rate of opening is voltage and calcium dependent. Fractal analysis of the P channels reveals that its behaviour does not seem to be fractal in nature. At voltages where only one conductance level is observed, fractal analysis shows at least one discrete open state and at least two discrete closed states. There are considerable similarities between the P channel and channels found in granules from the hypophysis. These channels resemble, in turn, the channels found in gap junctions. Therefore, it is not unwarranted to speculate that a gap‐junction‐like communication between the secretory vesicle and the extracellular space may occur during exocytosis.
UR - http://www.scopus.com/inward/record.url?scp=0024790439&partnerID=8YFLogxK
U2 - 10.1113/expphysiol.1989.sp003330
DO - 10.1113/expphysiol.1989.sp003330
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C2 - 2483274
AN - SCOPUS:0024790439
SN - 0144-8757
VL - 74
SP - 1019
EP - 1031
JO - Quarterly Journal of Experimental Physiology
JF - Quarterly Journal of Experimental Physiology
IS - 6
ER -