HSF and Msn2/4p can exclusively or cooperatively activate the yeast HSP104 gene

Melanie R. Grably, Ariel Stanhill, Osnat Tell, David Engelberg

نتاج البحث: نشر في مجلةمقالةمراجعة النظراء


In an effort to understand how an accurate level of stress-specific expression is obtained, we studied the promoter of the yeast HSP104 gene. Through 5′ deletions, we defined a 334 bp fragment upstream of the first coding AUG as sufficient and essential for maximal basal activity and a 260 bp fragment as sufficient and essential for heat shock responsiveness. These sequences contain heat shock elements (HSEs) and stress response elements (STREs) that cooperate to achieve maximal inducible expression. However, in the absence of one set of factors (e.g. in msn2Δmsn4Δ cells) proper induction is obtained exclusively through HSEs. We also show that HSP104 is constitutively derepressed in ras2Δ cells. This derepression is achieved exclusively through activation of STREs, with no role for HSEs. Strikingly, in ras2Δmsn2Δmsn4Δ cells the HSP104 promoter is also derepressed, but in this strain derepression is mediated through HSEs, showing the flexibility and adaptation of the promoter. Thus, appropriate transcription of HSP104 is usually obtained through cooperation between the Msn2/4/STRE and the HSF/HSE systems, but each factor could activate the promoter alone, backing up the other. Transcription control of HSP104 is adaptive and robust, ensuring proper expression under extreme conditions and in various mutants.

اللغة الأصليةالإنجليزيّة
الصفحات (من إلى)21-35
عدد الصفحات15
دوريةMolecular Microbiology
مستوى الصوت44
رقم الإصدار1
المعرِّفات الرقمية للأشياء
حالة النشرنُشِر - 2002
منشور خارجيًانعم


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