TY - JOUR
T1 - A monoclonal antibody specific for rat IL-18BP and its application in determining serum IL-18BP
AU - Maiti, Prasanta K.
AU - Im, Sin Hyeog
AU - Souroujon, Miriam C.
AU - Fuchs, Sara
N1 - Funding Information:
We thank Dr Daniela Novick for providing human IL-18BP. This research was supported by grants from The Association Francaise Contre les Myopathies, The Muscular Dystrophy Association of America and the Abramson Family Foundation.
PY - 2003/1/2
Y1 - 2003/1/2
N2 - IL-18 binding protein (IL-18BP) is a soluble inhibitory receptor for IL-18, a proinflammatory cytokine that plays a major role in IL-12-driven Th1 cell differentiation. IL-18BP is presumed to play a vital regulatory function in IL-18-mediated immune responses, although its precise role in immunomodulation in various diseases is not well understood. In the present study, we have generated and characterized a mouse monoclonal antibody (2A6H11), specific for rat IL-18BP. Its binding epitope is within amino acids 29-60 on IL-18BP and it does not interfere with the function of IL-18BP. Using this monoclonal antibody, we have developed an ELISA for determining IL-18BP levels. We observed elevated levels of IL-18BP in sera of rats with experimental autoimmune myasthenia gravis (EAMG), in comparison with the levels in healthy rats. Thus, this monoclonal antibody can serve as a valuable tool to elucidate the role of IL-18BP in EAMG and in other rat models for autoimmune diseases.
AB - IL-18 binding protein (IL-18BP) is a soluble inhibitory receptor for IL-18, a proinflammatory cytokine that plays a major role in IL-12-driven Th1 cell differentiation. IL-18BP is presumed to play a vital regulatory function in IL-18-mediated immune responses, although its precise role in immunomodulation in various diseases is not well understood. In the present study, we have generated and characterized a mouse monoclonal antibody (2A6H11), specific for rat IL-18BP. Its binding epitope is within amino acids 29-60 on IL-18BP and it does not interfere with the function of IL-18BP. Using this monoclonal antibody, we have developed an ELISA for determining IL-18BP levels. We observed elevated levels of IL-18BP in sera of rats with experimental autoimmune myasthenia gravis (EAMG), in comparison with the levels in healthy rats. Thus, this monoclonal antibody can serve as a valuable tool to elucidate the role of IL-18BP in EAMG and in other rat models for autoimmune diseases.
KW - Experimental myasthenia gravis
KW - Monoclonal antibody
KW - Rat IL-18 binding protein
UR - http://www.scopus.com/inward/record.url?scp=0037413506&partnerID=8YFLogxK
U2 - 10.1016/S0165-2478(02)00213-4
DO - 10.1016/S0165-2478(02)00213-4
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C2 - 12505199
AN - SCOPUS:0037413506
SN - 0165-2478
VL - 85
SP - 65
EP - 70
JO - Immunology Letters
JF - Immunology Letters
IS - 1
ER -